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Quercetin 7-Rhamnoside Reduces Porcine Epidemic Diarrhea Virus Replication Via Independent Pathway of Viral Induced Reactive Oxygen Species., Vol. 8

By Song, Jae Hyoung

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Book Id: WPLBN0004439780
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Reproduction Date: 2011-10-04

Title: Quercetin 7-Rhamnoside Reduces Porcine Epidemic Diarrhea Virus Replication Via Independent Pathway of Viral Induced Reactive Oxygen Species., Vol. 8  
Author: Song, Jae Hyoung
Volume: Vol. 8
Language: English
Subject: Physics, Terrorism
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Historic
Publication Date:
2011-10-04
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Hyoung, S. J. (2011-10-04). Quercetin 7-Rhamnoside Reduces Porcine Epidemic Diarrhea Virus Replication Via Independent Pathway of Viral Induced Reactive Oxygen Species., Vol. 8. Retrieved from http://community.worldlibrary.net/


Description
Description: This article is from Virology Journal, volume 8.

Abstract

Background: On the base of our previous study we were observed relevant studies on the hypothesis that the antiviral activity of quercetin 7-rhamnoside (Q7R), a flavonoid, won't relate ability of its antioxidant. Methods: We were investigated the effects of Q7R on the cytopathic effects (CPE) by CPE reduction assay. Production of DNA fragment and reactive oxygen species (ROS) induced by PEDV infection were studied using DNA fragmentation assay and flow cytometry. Results: In the course of this study it was discovered that Q7R is an extremely potent compound against PEDV. The addition of Q7R to PEDV-infected Vero cells directly reduced the formation of a visible cytopathic effect (CPE). Also, Q7R did not induce DNA fragmentation. Furthermore, ROS increased the infection of PEDV, which was strongly decreased by N-acetyl-L-cysteins (NAC). However, the increased ROS was not decreased by Q7R. Antiviral activity of antioxidants such as NAC, pyrrolidine dithiocarbamate (PDTC), and the vitamin E derivative, trolox, were hardly noticed. Conclusions: We concluded that the inhibition of PEDV production by Q7R is not simply due to a general action as an antioxidants and is highly specific, as several other antioxidants (NAC, PDTC, trolox) are inactive against PEDV infection.


 

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